Collection, analysis of difficult to manage activated immune cells ...

To study the profiles of gene expression and protein synthesis in neutrophils could reveal essential information on the immune response, but the collection of cells for the analysis was difficult. Isolation procedures standard last more than two hours and require blood samples relatively large. The neutrophils are also susceptible to manipulations and easily are activated, changing the molecular models of interest, and contain small amounts of messenger RNA, which is required for studies of gene expression.While laboratory tests were encouraging, samples from severely injured patients should be handled and processed in real clinical situations. Thanks to the efforts of the study co-author Lyle Moldawer, PhD, University of Florida College of Medicine, the devices were tested on six other sites participating in a national study of the immune response to injury. Analysis of samples from 26 patients with severe burns or other injuries revealed complex gene expression profiles that are awarded during the 28 days after injury, probably reflecting complex interactions between the components of the immune system.

Based on their experience in the development of devices based on silicon-chip capture of CD4 T cells for the diagnosis or isolate circulating tumor cells, Kotz?s team has developed a system that gathers a neutrophil-rich sample from the sample by a microliter of blood in less than 5 minutes, reducing the risk of disturbing cells in the process. To meet the requirements of speed and accuracy, researchers redesigned l ?, geometry antibody-based coating and other aspects of the module cell capture the heart of the device. The samples were able to reveal differences in the activity of the gene and protein relevant to the state of cellular activation.

Ronald Tompkins, MD, ScD ? Chief of the MGH Burns, a study co-author and principal investigator of the ?Inflammation and the host response to injury? initiative ? said: ?This technology has been widely implemented in our program,? Glue Grant ?, with a significant impact. The ability to capture specific cells in a clinical routine quickly and accurately provides a possible change in the paradigm of normal clinical diagnosis.? The study of Nature Medicine is part of the National Institutes of Health-funded, large collaborative research program and involved its seven clinical sites and seventeen academic institutions of the United States.

Part of the body?s first line of defense against injuries and infections, neutrophils have long been thought to play a fairly simple, such as releasing antimicrobial proteins and ingestion of pathogens. But recent studies find their actions to be more complex and critical for inflammation both acute and chronic, in particular the activation of the immune system in response to a lesion.

Our findings are of particular interest, given that about 40 percent of commercial disinfectants for surfaces on the market are alcohol or ammonia, said Professor John, who is also a researcher at the Institute of nutraceuticals and functional foods .

A team led by Massachusetts General Hospital scientists has developed a new microfluidic tool for quickly and accurately isolating neutrophils ? the most abundant type of white blood cells ? from small samples of blood, a result that could provide information essential to understanding the response of the immune system of a traumatic injury. The system, described in a paper in Nature Medicine, which received early online release, can also be adapted to isolate almost any type of cell.

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